Interesting links
With discovair we are developing the lung for the human cell atlas. As they describe it themselves: "To create comprehensive reference maps of all human cells—the fundamental units of life—as a basis for both understanding human health and diagnosing, monitoring, and treating disease." If you want to visit the Human Cell Atlas, or other interesting reads or find more about our data, see the links below:
General
Data repositories
Protocols
Cell dissociation of fresh human lung tissue for single-cell RNA-seq
Ilias Angelidis, Maximilian Strunz, Herbert Schiller
Link to ProtocolCell dissociation from nasal and bronchial brushings with cold-active protease for single-cell RNA- seq V.2
Laure-Emmanuelle Zaragosi, Pascal Barbry
Link to ProtocolCell dissociation from airway biopsies with cold-active protease for single-cell RNA-seq
Laure-Emmanuelle Zaragosi, Pascal Barbry
Link to ProtocolSingle-cell suspension preparation from Human bronchial biopsies to perform scRNA-sequencing using 10x chromium
Martijn Nawijn, Leonie Apperloo
Link to ProtocolCell dissociation from nasal, bronchial and tracheal brushings with cold-active protease for single-cell RNA-seq
Kaylee B Worlock, Masahiro Yoshida, Kerstin Meyer, Marko Z. Nikolić
Link to ProtocolSCRINSHOT V.2
Alexandros Sountoulidis, Andreas Liontos, Hong Phuong Nguyen, Alexandra Firsova, Athanasios Fysikopoulos, Xiaoyan Qian, Werner Seeger, Erik Sundström, Mats Nilsson, Christos Samakovlis
Link to ProtocolIn situ sequencing for RNA analysis in tissue sections V.2
Chika Yokota, Daniel Gyllborg, Mats Nilsson
Link to ProtocolCGAP Human Lung Dissociation - Tissue Stability Study
Anna Wilbrey-Clark, Adam Hunter
Link to Protocol